Biotinylated mouse tim4-fc
WebOct 24, 2007 · Mouse Tim4 is a type I membrane protein, consisting of a signal sequence and extracellular, ... Tim4-Fc-coated latex beads incubated with Tim1-Fc in the absence … WebMonoclonal Hamster‑Armenian anti‑Mouse TIMD4 / TIM4 / TIM‑4 Antibody (FITC, IF) LS‑C663029. ... FC: Cells were incubated on ice for 30 min with 1 ug/ml biotinylated Kat5-18 in 100 ul staining solution (PBS containing 2% FCS), followed by incubation with 2 ug/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 ug/ml ...
Biotinylated mouse tim4-fc
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WebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT … WebRegarding Tim4 expression, cells were stained with PE-labeled cIg, or RMT4-54 (anti-Tim4: BioLegend). Mouse peritoneal resident cells were pretreated with clone 93 (anti-CD16/32: BioLegend) to block Fc receptors, and then stained with FITC-anti-CD11b (BioLegend) and the above mAbs. These cells were analyzed by an Accuri C6 cytometer (BD ...
WebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these … WebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg ...
WebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … WebJun 29, 2024 · An ELISA plate (AGC Techno Glass, Shizuoka, Japan) was coated with 1 μg/mL recombinant mouse TIM4-Fc protein (FUJIFILM Wako Pure Chemical)/50 mM carbonate-bicarbonate buffer (pH 9.6) and ...
WebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM …
WebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This … fitbit flex automatic sleep trackingWebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. can ford unlock my vehicle remotelyWebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, canford weddingsWebCD45 in Mouse Thymus. CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # … can ford sync 3 be updatedWebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with … can ford sync unlock my carWebMar 27, 2005 · The newly identified TIM family of proteins is associated with regulation of T helper type 1 (TH1) and TH2 immune responses. TIM-1 is genetically linked to asthma and is a receptor for hepatitis A ... fitbit flex activity sleep trackerWebWe offer Anti-Mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F (ab') 2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols. can ford sync be upgraded