Detritylation什么意思
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Detritylation什么意思
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Web除毛. 脫毛 脫毛法 拔毛術. 脫毛作用. "chemical depilation" 中文翻譯 : 化學脫毛法. "depilation agent" 中文翻譯 : 脫毛劑. "depilation by lime" 中文翻譯 : 浸灰脫毛法. "electrolytic … WebMay 13, 2024 · 通常，先利用一定浓度的盐去无DMT保护的杂质，然后在柱上利用酸洗脱三苯甲基（Detritylation），最后利用不同的盐浓度把目的寡核苷酸分离纯化。 纯化后的 …
WebIt is challenging to effectively deprotect hydroxyl groups of acid-or-base sensitive bio-macromolecules without causing even minor defects and compromising high quality of final products. We report here a mild detritylation strategy in mildly acidic buffers to remove the DMTr protection from the 5'-hydroxyl groups of synthetic nucleic acids. http://www.iciba.com/word?w=deviation#:~:text=A%20minimal%20error%20or%20deviationmay,result%20in%20wide%20divergence.%20%E5%A4%B1%E4%B9%8B%E6%AF%AB%E5%8E%98%2C%E8%B0%AC%E4%BB%A5%E5%8D%83%E9%87%8C.
Webdefloration是什么意思？defloration怎么读？新东方在线字典为用户提供单词defloration的释义、defloration的音标和发音、defloration的用法、例句、词组、词汇搭配、近反义词等内 … WebNov 2, 2024 · When 3% TCA was used in the detritylation step, studies show that acid delivery time as short as 10 seconds (versus 110 seconds) did not significantly compromise the yield of the full-length product. 3 A deblocking step that completes in less than one minute is ideal for reducing the oligonucleotide’s exposure to acid. 4.
Webaccuracy are increased by this wash, since premature detritylation of the incoming phosphoramidite monomer is prevented. Activation/Coupling Following deblocking of the 5′ hydroxyl group, the next protected phosphoramidite is delivered to the reaction column along with the weakly acidic activator tetrazole (pK a = 4.8).
Webn. 定界，划界；区划。. "agroclimatic delimitation"中文翻译 农业气候分区. "cell delimitation"中文翻译 信元定界. "delimitation of a frontier"中文翻译 边境分界. … reading body language booksWebDetritylation. Detritylation, phosphorylation of the free hydroxyl group with chlorodimethyl phosphate, and hydrogenolysis provided the desired Man-6-P derivative which was … how to strengthen your elbowWebA move made in opposition or retaliation to another. 反作用用来反对或缓和一种作用的作用. 期刊摘选. Like retaliation tariff, preferential tariff is used as a weapon in international … how to strengthen your hairWebAug 1, 1996 · Abstract. Under the conditions normally used for detritylation in oligonucleotide synthesis, the haloacetic acid binds strongly to the oligonucleotide. … reading body partsWebNov 3, 2024 · Verb [ edit] detritylate ( third-person singular simple present detritylates, present participle detritylating, simple past and past participle detritylated ) To modify, or be modified, by detritylation. This page was last edited on 3 November 2024, at 06:24. how to strengthen your gripWebThe understanding, control, and removal of nonoligonucleotide process-related impurities (PRI) are of key importance for the manufacturing of therapeutic oligonucleotides as their presence in the final product is both a quality and safety concern. Regulatory agencies require manufacturers to demonstrate that PRI are under control or adequately purged … how to strengthen your hamstringWebIf needed, the final detritylation step cleaves the terminal 5′ DMT group from the oligonucleotide. The process requires acidic conditions and can be conducted in aqueous media in a vessel, or directly on the purification column. 26, 27 The main byproducts of the reaction are DMT alcohol (DMT-OH) and the acid used (e.g., acetic acid), which ... reading body language cues